Premila Abraham

RESEARCH INTERESTS

1. Mechanism of anti -cancer drug induced organ toxicity and its prevention

• Methotrexate induced gastrointestinal toxicity

• Cyclophosphamide induced hemorrhagic cystitis

2.  Targeting endometrial cancer stem cells for the treatment of endometrial cancer using aspirin and curcumin

3.  Mechanism of hypoglycemic and hypolipidemic effects of mega doses of biotin in rat model of gestational diabetes

RESEARCH GRANTS

Publications

1. Muthuraman N, Anitha Thomas, Thomas Samuel Ram, Mohankumar KM, Abraham P (2025). "Is There Any Difference in Stem Cell Population between Type I and Type II Endometrial Cancer? A Pilot Study" Journal of Mother and Child, vol. 29, no. 1, Sciendo, 2025, pp. 10-19. https://doi.org/10.34763/jmotherandchild.20252901.d-24-00041

2. N. Muthuraman, Abraham P (2024). Serum Tumour Necrosis Factor-α (TNF-α) and Lipid Profile in Gestational Diabetes Mellitus. Journal of Obstetrics and Gynaecology Canada, Volume 46, Issue 8, 2024, 102592, ISSN 1701-2163, https://doi.org/10.1016/j.jogc.2024.102592

3. Hemalatha R, Muthuraman N, Sandya Rani B, Abraham P (2023). A study on the alteration of endoplasmic reticulum stress related proteins in cyclophosphamide induced damage to urothelium. Pharm Biomed Res 2023; 9 (2) :153-162

4. Muthuraman N, Vijayselvi R, Sudhakar P Y, Christudoss P, Abraham P (2023). Assessment of serum biotin levels and its association with blood glucose in gestational diabetes mellitus. Eur J Obstet Gynecol Reprod Biol X. 2023;17:100181. Published 2023 Feb 17. doi:10.1016/j.eurox.2023.100181 

5. Ramamoorthy H, Abraham P, Isaac B (2021). Melatonin protects against tenofovir-induced nephrotoxicity in rats by targeting multiplecellular pathways. Hum Exp Toxicol. 2021 May;40(5):826-850. doi: 10.1177/0960327120968860. Epub 2020 Nov 4. PMID: 33146023.

6. Rammamoorthy H, Abraham P (2019). Mitochondrial pathway of apoptosis and necrosis contribute to tenofovir disoproxil fumarate -induced renal damage in rats. Hum Exp Toxicol. 2019 Mar;38(3):288-302. doi: 10.1177/0960327118802619. Epub 2018 Oct 16

7. Natarajan K, Abraham P, Kota R, Isaac B (2018). NF-κB-iNOS-COX2-TNF α inflammatory signaling pathway plays an important role in methotrexate induced small intestinal injury in rats. Food Chem Toxicol. 2018 Aug;118:766-783. doi: 10.1016/j.fct.2018.06.040. Epub 2018 Jun 20.PMID: 29935243

8. Ramamoorthy H, Abraham P, Isaac B, Selvakumar D (2017). Role for NF-κB inflammatory signalling pathway in tenofovir disoproxil fumarate (TDF) induced renal damage in rats. Food Chem Toxicol. 2017 Jan;99:103-118. doi: 10.1016/j.fct.2016.11.029. Epub 2016 Nov 27. PMID: 27899301.

9. Abraham P, Hemalatha Ramamoorthy, Deepak Vinod Francis, Suganthy Rabi (2017). Beneficial effects of megadoses of biotin in Streptozotocin-induced gestational diabetes mellitus in rats – A preliminary study. Indian J Physiol Pharmacol. 61:159-165.

10. Natarajan K, Abraham P, Kota R, Selvakumar D (2017). Aminoguanidine pretreatment prevents methotrexate-induced small intestinal injury in the rat by attenuating nitrosative stress and restoring the activities of vital mitochondrial enzymes.J Basic Clin Physiol Pharmacol. 2017 May 1;28(3):239-247. doi: 10.1515/jbcpp-2016-0099. PMID: 28099126.

11. Natarajan K, Abraham P, Kota R (2017). Activation of the mitochondrial apoptotic pathway contributes to methotrexate-induced small intestinal injury in rats.Cell Biochem Funct. 2017 Oct;35(7):378-391. doi: 10.1002/cbf.3285. Epub 2017 Sep 4. PMID: 2887159

12. Abraham P, Deepak Vinod Francis, Suganthy Rabi (2016). Increased hepatic gluconeogenesis and decreased glucose uptake, and increased hepatic de novo lipogenesis in rat model of maternal diabetes. Biomedical Research 27:632-640.

13. Natarajan K, Abraham P, Isaac B (2016). Effect of methotrexate treatment on iNOS gene expression, protein tyrosine nitration protein cysteine nitrosylation, and the activities of mitochondrial enzymes in the small intestinal mucosa of rats. Asian Journal of Pharmacology and Toxicology. July 2016.

14. Natarajan K, Abraham P(2016). Methotrexate administration induces differential and selective protein tyrosine nitration and cysteine nitrosylation in the subcellular organelles of the small intestinal mucosa of rats. Chem Biol Interact. 2016 May 5;251:45-59. doi: 10.1016/j.cbi.2016.03.032. Epub 2016 Mar 30. PMID: 27038877.

15. Abraham P, Bina Isaac (2016). Imbalance Between Peroxisomal ROS Generating Enzymes and ROS Scavenging Enzymes May Contribute to Cyclophosphamide Induced Renal Damage in Rats Indian Journal of Applied Research Volume : 6 | Issue : 11 | November 2016, 72-77

16. Abraham P, R. Hemalatha, Bina Isaac (2015).A reliable and reproducible rodent model of Tenofovir disoproxil fumarate (TDF) (anti-HIV drug) nephrotoxicity that resembles human TDF tubulopathy. Biomedical Research 2015; 27(1):974-982.

17. Kolli VK, Natarajan K, Isaac B, Selvakumar D, Abraham P(2014). Mitochondrial dysfunction and respiratory chain defects in a rodent model of methotrexate- induced enteritis. Hum Exp Toxicol. 2014 Oct;33(10):1051-65. doi: 10.1177/0960327113515503. Epub 2013 Dec 17. PMID: 24347301.

18. Hemalatha Ramamoorthy, Abraham P and Bina Isaac (2014). Mitochondrial dysfunction and electron transport chain complex defect in a rat model of tenofovir disoproxil fumarate nephrotoxicity. Preclinical efficacy of melatonin in the amelioration of tenofovir nephrotoxicity by the attenuation of oxidative stress, nitrosative stress and inflammation in rats. J Basic Clin Physiol Pharmacol 2014; 25(4): 387– 399

19. Ramamoorthy H, Isaac B, Abraham P (2014). Tenofovir induced renal damage is associated with activation of NF-κB inflammatory signaling pathway and PARP overactivation. BMC Infectious Diseases. 2014;14(Suppl 3): E5.

20. Faith M, Abraham P (2013). An update on diagnostic value of biotinidase: From liver damage to cancer: Minireview. Biomedical Research 2013; 24 (2).267-273

21. Kolli VK, Kanakasabapathy I, Faith M, Ramamoorthy H, Isaac B, Natarajan K, Abraham P (2013). A preclinical study on the protective effect of melatonin against methotrexate-induced small intestinal damage: effect mediated by attenuation of nitrosative stress, protein tyrosine nitration, and PARP activation. Cancer Chemother Pharmacol. 2013 May;71(5):1209-18. doi: 10.1007/s00280-013-2115-z. Epub 2013 Feb 19. PMID: 23420439.

22. Kolli VK, Abraham P, Isaac B, Kasthuri N (2013). Preclinical efficacy of melatonin to reduce methotrexate-induced oxidative stress and small intestinal damage in rats. Dig Dis Sci. 2013 Apr;58(4):959-69. doi: 10.1007/s10620-012-2437-4. Epub 2012 Oct 10. PMID: 23053903.

23. Abraham P, Ramamoorthy H, Isaac B (2013). Depletion of the cellular antioxidant system contributes to tenofovir disoproxil fumarate -induced mitochondrial damage and increased oxido-nitrosative stress in the kidney.J Biomed Sci. 2013 Aug 19;20(1):61. doi: 10.1186/1423-0127-20-61. PMID: 23957306; PMCID: PMC3765371.

24. Abraham P, Issac B, Ramamoorthy H(2012). Evidence for the roles of oxidative stress, nitrosative stress and Nf-Kb activation in Tenofovir Disoproxil Fumarate (TDF) induced renal damage in rats.BMC Infectious Diseases 2012, 12(Suppl 1):P6

25. Abraham P, Isaac B(2011). Ultrastructural changes in the rat kidney after single dose of cyclophosphamide--possible roles for peroxisome proliferation and lysosomal dysfunction in cyclophosphamide-induced renal damage. Hum Exp Toxicol. 2011 Dec;30(12):1924-30. doi: 10.1177/0960327111402240. Epub 2011 Mar 18. PMID: 21421693

26. Abraham P, Isaac B(2011). The effects of oral glutamine on cyclophosphamide-induced nephrotoxicity in rats.Hum Exp Toxicol. 2011 Jul;30(7):616-23. doi: 10.1177/0960327110376552. Epub 2010 Jul 9.PMID: 20621952.

27. Abraham P, Suganthy Rabi (2011). Protective effect of aminoguanidine against cyclophosphamide induced oxidative stress and renal damage in rats. Redox Rep. 2011;16(1):8-14. doi: 10.1179/174329211X12968219310837.

28. Abraham P, Rabi S (2011). Aminoguanidine, a Selective Nitric Oxide Synthase Inhibitor, attenuates Cyclophosphamide induced renal damage by Inhibiting Protein Nitration and PARP(S) Activation. Chemotherapy. 2011;57(4):327-34. doi: 10.1159/000330463. Epub 2011 Sep 1. PMID: 21893984.

29. Ramamoorthy H, Abraham P, Isaa, B(2011).Oxidative stress, decreased activities of anti-oxidant enzymes, and neutrophil infiltration contribute to Tenofovirdisoproxil fumarate induced renal damage in rats. International Research Journal of Pharmacy and Pharmacology 2011; 1(10) :259-270.

30. Abraham P, Isaac B, Ramamoorthy H, Natarajan K(2011). Oral glutamine attenuates cyclophosphamide-induced oxidative stress in the bladder but does not prevent hemorrhagic cystitis in rats. J Med Toxicol. 2011 Jun;7(2):118-24. doi: 10.1007/s13181-010-0103-9. PMID: 20661687; PMCID: PMC3724431.

31. Abraham P, Kolli VK, Rabi S(2010). Melatonin attenuates methotrexate-induced oxidative stress and renal damage in rats. Cell Biochem Funct. 2010 Jul;28(5):426-33. doi: 10.1002/cbf.1676. PMID: 20589739.

32. Abraham P, Rabi S, Selvakumar D(2009).Protective effect of aminoguanidine against oxidative stress and bladder injury in cyclophosphamide-induced hemorrhagic cystitis in rat.Cell Biochem Funct. 2009 Jan;27(1):56-62. doi: 10.1002/cbf.1534. PMID: 19107871.

33. Kolli VK, Abraham P, Isaac B, Selvakumar D(2009). Neutrophil infiltration and oxidative stress may play a critical role in methotrexate-induced renal damage.Chemotherapy. 2009;55(2):83-90. doi: 10.1159/000192391. Epub 2009 Jan 15. PMID:19145077.

34. Abraham P, Rabi S, Kulothungan P(2009). Aminoguanidine, selective nitric oxide synthase inhibitor,ameliorates cyclophosphamide-induced hemorrhagic cystitis by inhibiting protein nitration and PARS activation. Urology. 2009 Jun;73(6):1402-6. doi: 10.1016/j.urology.2008.10.039. Epub 2009 Feb 28. PMID: 19251306.

35. Abraham P, Rabi S(2009). Protein nitration, PARP activation and NAD+ depletion may play a critical role in the pathogenesis of cyclophosphamide-induced hemorrhagic cystitis in the rat. Cancer Chemother Pharmacol. 2009 Jul;64(2):279-85. doi: 10.1007/s00280-008-0868-6. Epub 2008 Nov 18. PMID: 19015854.

36. Abraham P, Rabi S(2009). Nitrosative stress, protein tyrosine nitration, PARP activation and NAD depletion in the kidneys of rats after single dose of cyclophosphamide. Clin Exp Nephrol. 2009 Aug;13(4):281-287. doi: 10.1007/s10157-009-0160-z. Epub 2009 Mar 7. PMID: 19266253.

37. Abraham P, Sugumar E(2008). Increased glutathione levels and activity of PON1 (phenyl acetate esterase) in the liver of rats after a single dose of cyclophosphamide: a defense mechanism? Exp Toxicol Pathol. 2008 Mar;59(5):301-6. doi: 10.1016/j.etp.2007.06.006. Epub 2007 Nov 26. PMID: 18032000.

38. Abraham P, Sugumar E(2008). Enhanced PON1 activity in the kidneys of cyclophosphamide treated rats may play a protective role as an antioxidant against cyclophosphamide induced oxidative stress. Arch Toxicol. 2008 Apr;82(4):237-8. doi: 10.1007/s00204-007-0240-3. Epub 2007 Sep 14. PMID: 17874068.

39. Abraham P, Indirani Kanakasabapathy , Preethi kulothungan(2008).Alterations in antioxidant enzyme activities and increased oxidative stress in cyclophosphamide induced hemorrhagic cystitis in the rat.Cancer Therapy 6: 563-570.

40. Kolli VK, Abraham P, B. Isaac(2008). Alteration in antioxidant defense mechanisms in the small intestines of methotrexate treated rat may contribute to its gastrointestinal toxicity. Cancer Therapy 2008. 5, 501-510

41. Kolli VK, Abraham P, Rabi S(2008). Methotrexate-induced nitrosative stress may play a critical role in small intestinal damage in the rat. Arch Toxicol. 2008 Oct;82(10):763-70. doi: 10.1007/ s00204-008-0287-9. Epub 2008 Feb 6. PMID: 18253714.

42. Abraham P, Ramakrishna B (2007). Oxidative stress plays a role in the pathogenesis of acetaminophen induced liver damage in the rat. Research and Reviews in Biosciences 1:53-58.

43. Sugumar E, Indirani K, Abraham P (2007). Normal plasma creatinine levels despite histological evidence of renal damage in cyclophosphamide-treated rats. Clin Chim Acta. 2007 Feb;376(1-2):244-5. doi: 10.1016/j.cca.2006.04.006. Epub 2006 Jun 5.

44. Abraham P, Indirani K, Sugumar E(2007). Effect of cyclophosphamide treatment on selected lysosomal enzymes in the kidney of rats.Exp Toxicol Pathol. 2007 Oct;59(2):143-9. doi: 10.1016/j.etp.2007.05.003. Epub 2007 Aug 7. PMID: 17686619.

45. Abraham P, Indirani K, Sugumar. E(2007)..Decrease in the activities of lysosomal enzymes may contribute to the urotoxicity of cyclophosphamide in the rat . Biomedical Research 2007; 18:20-27. https://www.biomedres.info/

46. Vyas R, Satishkumar S, Selvaraj KG, Suganthy J, Faith M, Isaac B and Abraham P (2006). Teaching in basic sciences- a novel approach integrated case-based learning. CMC Alumni Journal 40:11-15.

47. Dhanarajan R, Abraham P, B. Isaac(2006). Protective effect of ebselen, a selenoorganic drug , against gentamicin –induced renal damage in rats. Basic Clin Pharmacol Toxicol, 2006.Sep;99(3): 267-72.

48. Abraham P, K I, K D (2005). Nitro Arginine Methyl ester, a non selective inhibitor of nitric oxide synthase reduces ibuprofen induced gastric mucosal injury in the rat. Dig Dis Sci. 2005 Sep;50(9):1632-40. doi: 10.1007/s10620-005-2908-y. PMID: 16133962

49. Abraham P (2005)..Oxidative stress in paracetamol-induced pathogenesis:(I)renal damage. Indian J Biochem Biophys. 2005 Feb;42(1):59-62.

50. Abraham P (2005)..Vitamin C may be beneficial in the prevention of paracetamol induced renal damage . Clin Exp Nephrol. 2005 Mar;9(1):24-30. doi: 10.1007/s10157-004-0335-6. PMID: 15830269.

51. Abraham P (2005). Megadose of vitamin C augments the nephrotoxicity of paracetamol . Nephrology (Carlton). 2005 Dec;10(6):623-4. doi: 10.1111/j.1440-1797.2005.00477.x. PMID: 16354249.

52. Abraham P, Kanakasabapathy I, Dian BJ(2005). Propylthiouracil attenuates acetaminophen-induced renal damage in the rat.Nephrology (Carlton). 2005 Dec;10(6):588-93. doi: 10.1111/j.1440-1797.2005.00494. x. PMID:16354242.

53. Abraham P (2004).Increased plasma biotinidase activity in rats with paracetamol induced acute liver injury . Clin Chim Acta. 2004 Nov;349(1-2):61-5. doi: 10.1016/j.cccn.2004.06.002. PMID: 15469856.

54. Abraham P (2004). Increased protein thiol in the liver and plasma of rats in acute acetaminophen intoxication. Clin Chim Acta. 2004 Jan;339(1-2):203-4. doi: 10.1016/j.cccn.2003.09.022. PMID: 14687912.

55. Abraham P (2004). Lysosomal enzyme activity during development of carbon tetrachloride induced cirrhosis in rats.Indian J Physiol Pharmacol. 2004 Apr;48(2):206-12. PMID: 15521560.

56. Abraham P, Wilfred G (2003). A marked decrease in the activity of biotinidase in the plasma of rats after single dose of carbon tetrachloride. Clin Chim Acta. 2003 Feb;328(1-2):195-6. doi: 10.1016/s0009-8981(02)00381-9. PMID: 12559618.

57. Abraham P, Wilfred G, Ramakrishna B (2003). Decrease in plasma biotinidase activity with normal albumin concentrations in experimental liver fibrosis. Clin Chim Acta. 2003 Aug;334(1-2):245-7. doi: 10.1016/s0009-8981(03)00195-5. PMID:12867299.

58. Abraham P, Wilfred G (2002). A massive increase in serum β-glucuronidase after a single dose of carbon tetrachloride to the rat. Clin Chim Acta. 2002 Aug;322(1-2):183-4. doi: 10.1016/s0009-8981(02)00170-5. PMID: 12104100

59. Abraham P, Wilfred G, Ramakrishna B (2002). Oxidative damage to the hepatocellular proteins after chronic ethanol intake in the rat. Clin Chim Acta. 2002 Nov;325(1-2):117-25. doi: 10.1016/s0009-8981(02)00279-6. PMID: 12367775.

60. Abraham P, Wilfred G (2001). Oxidative damage to the lipids and proteins of the lungs of rats upon acute exposure to carbon tetrachloride vapours.(2001) International Journal of Medical Toxicology and Legal Medicine 3: 22:16-17.

61. Abraham P, Wilfred G (2000). Lysosomal enzymes in the pathogenesis of carbon tetrachloride induced injury to the kidney and testis in the rat. Indian Journal of Pharmacology 32: 250-251.

62. Abraham P, Wilfred G, Ramakrishna B (2000). Plasma prolidase may be an index of liver fibrosis in the rat. Clin Chim Acta. 2000 May;295(1-2):199-202. doi: 10.1016/s0009-8981(00)00183-2. PMID: 10928820

63. Abraham P, Wilfred G, Ramakrishna B (1999). Effect of administration of the free radical trapping agent “N-tert-Butyl-Alpha-Phenylnitrone” on the prevention of oxidative damage to lipids and proteins of the liver and liver damage in experimental cirrhosis. Journal of Forensic Medicine and Toxicology1999; 16: 26-29

64. Abraham P, Wilfred G, Ramakrishna B(1999). Decreased activity of hepatic alkaline protease in rats with carbon tetrachloride-induced liver cirrhosis. Indian J Exp Biol. 1999 Dec;37(12):1243-4. PMID: 10865892.

65. Abraham P, Wilfred G, Cathrine(1999). Oxidative damage to the lipids and proteins of the lungs, testis and kidney of rats during carbon tetrachloride intoxication. Clin Chim Acta. 1999 Nov;289(1-2):177-9. doi: 10.1016/s0009-8981(99)00140-0. PMID: 10636716.

66. Sundari PN (Abraham P), Wilfred G, Ramakrishna (1997). Does oxidative protein damage play a role in the pathogenesis of carbon tetrachloride-induced liver injury? Biochim.Biophys. Acta.1997;1362: 169- 76.

CHAPTERS IN BOOKS

1. Premila Abraham, Bina Isaac. Protective Effects of Exogenous Melatonin Against Methotrexate-Induced Intestinal Injury October 2014DOI10.1007/978-81-322-0825-9_29In book: Melatonin and Melatonergic Drugs in Clinical Practice Melatonin and Melatonergic Drugs in Clinical Practice chapter 29; pp 389-402. 

AWARDS

1. PMSDS (Pharma Medical Sciences Development Society) Excellence in Research Award 2021 for outstanding contribution in the field of Medical Biochemistry

2. DBT National Women Scientist award 2010

3. DBT Crest International fellowship Award 2011

4. ICMR – Shakuntala Amirchand prize award 2005

5. The Tamil Nadu Young Womens’ scientist award 2004